NF-kB p65 ELISA Kit

Produktnummer ABIN8126254

Das Human NF-kB p65 ELISA Kit (ABIN8126254) ist ein Colorimetric ELISA Kit zur Detektion von Human NF-kB p65.

Kurzübersicht für NF-kB p65 ELISA Kit (ABIN8126254)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.313 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails NF-kB p65 ELISA Kit

Untere Nachweisgrenze: 0.313 ng/mL

Verwendungszweck: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytische Methode: Quantitative

Sensitivität: 0.188 ng/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human NF-kB p65 concentrations in serum, plasma. It also detects cytoplasmic, nuclear and total NF-kB p65 from cells. Detection Range: 0.313 - 20 ng/mL Sensitivity: < 0.188 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human p65 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human p65 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human p65 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human p65 can be calculated using the standard curve. Kit Components

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: Please refer to the manual

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: NF-kB p65 (Nuclear Factor Kappa B p65)

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine