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NF-kB p65 ELISA Kit

NFkBP65 Reaktivität: Human, Ratte, Maus pSer536 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN2748433
  • Target Alle NF-kB p65 (NFkBP65) ELISA Kits anzeigen
    NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
    Bindungsspezifität
    pSer536, total
    Reaktivität
    • 24
    • 19
    • 14
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Ratte, Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human/Mouse/Rat Phospho-NF-KB p65 (S536) ELISA and Total NF-KB p65 Kit. This assay semi-quantitatively measures phophorylated NF-KB p65 (Ser536) and Total NF-KB p65 in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human NF-KB p65 phosphorylated at site Serine-536 and Total NF-KB p65.
    Produktmerkmale
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle NF-kB p65 (NFkBP65) ELISA Kits anzeigen
    NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
    Andere Bezeichnung
    NFKBP65 (NFkBP65 Produkte)
    Synonyme
    C-Rel ELISA Kit, NFKB3 ELISA Kit, p65 ELISA Kit, NFkB ELISA Kit, c-rel ELISA Kit, zgc:100833 ELISA Kit, Xrel2 ELISA Kit, Xrel3 ELISA Kit, rel ELISA Kit, rel-A ELISA Kit, rel2 ELISA Kit, rel3 ELISA Kit, v-rel ELISA Kit, xrel ELISA Kit, REL proto-oncogene, NF-kB subunit ELISA Kit, RELA proto-oncogene, NF-kB subunit ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A (avian) ELISA Kit, v-rel avian reticuloendotheliosis viral oncogene homolog ELISA Kit, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog ELISA Kit, REL ELISA Kit, RELA ELISA Kit, Rela ELISA Kit, Rel ELISA Kit, rel ELISA Kit, rel.L ELISA Kit
    Hintergrund
    Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-KB p65) phosphorylated at Serine-536 and total NF-KB p65.
    Gen-ID
    5970
    UniProt
    Q04206
    Pathways
    NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine
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