MEK1 ELISA Kit

Produktnummer ABIN6730500

Produktdetails MEK1 ELISA Kit

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse MAP2K1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse MAP2K1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Andere Bezeichnung: MAP2K1 (MAP2K1 Produkte)

Synonyme: Mek1 ELISA Kit, CFC3 ELISA Kit, MAPKK1 ELISA Kit, MEK1 ELISA Kit, MKK1 ELISA Kit, PRKMK1 ELISA Kit, MEKK1 ELISA Kit, Prkmk1 ELISA Kit, mek-2 ELISA Kit, si:ch211-242m18.2 ELISA Kit, wu:fj56a12 ELISA Kit, wu:fj61b01 ELISA Kit, zgc:56557 ELISA Kit, ATMEK1 ELISA Kit, F20B18.180 ELISA Kit, F20B18_180 ELISA Kit, MAP kinase/ ERK kinase 1 ELISA Kit, MITOGEN ACTIVATED PROTEIN KINASE KINASE ELISA Kit, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 ELISA Kit, NMAPKK ELISA Kit, MEK ELISA Kit, mitogen activated protein kinase kinase 1 ELISA Kit, mitogen-activated protein kinase kinase 1 ELISA Kit, mitogen-activated protein kinase kinase 1 L homeolog ELISA Kit, MAP kinase/ ERK kinase 1 ELISA Kit, Map2k1 ELISA Kit, MAP2K1 ELISA Kit, map2k1.L ELISA Kit, map2k1 ELISA Kit, MEK1 ELISA Kit

UniProt: P31938

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling