AKT1 FLISA Kit
Kurzübersicht für AKT1 FLISA Kit (ABIN5691895)
Target
Alle AKT1 FLISA Kits anzeigenBindungsspezifität
Reaktivität
Nachweismethode
Methodentyp
Applikation
Proben
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Verwendungszweck
- The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.
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Analytische Methode
- Qualitative
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Produktmerkmale
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Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma -
Bestandteile
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2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual
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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA Kit
AKT1 Reaktivität: Human, Maus, Ratte pSer473 Colorimetric Sandwich ELISA Cell Lysate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 Reaktivität: Human, Maus, Ratte pSer473 Colorimetric Sandwich ELISA Cell Lysate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 Reaktivität: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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Applikationshinweise
- Detection Range: At least 5000 cells/well
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Testdauer
- 4.5 h
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Plattentyp
- Uncoated
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- 4°C/6 Months
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Haltbarkeit
- 6 months
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- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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Andere Bezeichnung
- Akt1
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Gen-ID
- 207
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UniProt
- P31749
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Pathways
- PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt
Target Alle AKT1 FLISA Kits anzeigen
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