Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

RIPK1 ELISA Kit

RIPK1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5658379
  • Target Alle RIPK1 ELISA Kits anzeigen
    RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))
    Reaktivität
    • 3
    • 2
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Receptor Interacting Serine Threonine Kinase 1 (RIPK1). No significant cross-reactivity or interference between Receptor Interacting Serine Threonine Kinase 1 (RIPK1) and analogues was observed.
    Sensitivität
    0.055 ng/mL
    Featured
    Zu unserem meistverkauften RIPK1 ELISA Kit
    Top Product
    Discover our top product RIPK1 ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 1 (RIPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor Interacting Serine Threonine Kinase 1 (RIPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 1 (RIPK1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 1 (RIPK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle RIPK1 ELISA Kits anzeigen
    RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))
    Andere Bezeichnung
    Receptor Interacting Serine Threonine Kinase 1 (RIPK1 Produkte)
    Synonyme
    RIP ELISA Kit, RIP1 ELISA Kit, D330015H01Rik ELISA Kit, Rinp ELISA Kit, Rip1 ELISA Kit, rip1 ELISA Kit, ripk1 ELISA Kit, xRIP1beta ELISA Kit, xrip1 ELISA Kit, receptor interacting serine/threonine kinase 1 ELISA Kit, receptor (TNFRSF)-interacting serine-threonine kinase 1 ELISA Kit, receptor interacting serine/threonine kinase 1 L homeolog ELISA Kit, RIPK1 ELISA Kit, Ripk1 ELISA Kit, ripk1.L ELISA Kit
    Hintergrund

    Gene Name: Receptor Interacting Serine Threonine Kinase 1

    Gene Aliases: RIP, Receptor-Interacting Protein, Cell death protein RIP, Serine/threonine-protein kinase RIP

    Gen-ID
    306886
    UniProt
    D3ZYL0
    Pathways
    NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interaktom, Ubiquitin Proteasome Pathway
Sie sind hier:
Kundenservice