p53 ELISA Kit

Produktnummer ABIN5520904

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human p53. Es ist 1 Publikation verfügbar.

Kurzübersicht für p53 ELISA Kit (ABIN5520904)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.125 pg/mL - 5000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails p53 ELISA Kit

Untere Nachweisgrenze: 78.125 pg/mL

Verwendungszweck: For quantitative detection of TP53 in serum, plasma, tissue homogenates.

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of TP53. No significant cross-reactivity or interference between TP53 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between TP53 and all the analogues, therefore, cross reaction may still exist.

Sensitivität: 46.875 pg/mL

Bestandteile: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Sandwich ELISA, Double Antibody

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Haltbarkeit: 6 months

Referenzen für p53 ELISA Kit (ABIN5520904)

Yuniarti, Mustofa, Aryandono, Haryana et al.: "Synergistic Action of 1,2-Epoxy-3 (3- (3,4-dimethoxyphenyl)- 4H-1-benzopiyran-4-on) Propane with Doxorubicin and Cisplatin through Increasing of p53, TIMP-3, and MicroRNA-34a in Cervical Cancer Cell ..." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 19, Issue 10, pp. 2955-2962, (2019) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: TP53

Hintergrund: TP53/BCC7/LFS1/TRP53/Antigen NY-CO-13/FLJ92943/LFS1TRP53/p53/p53 tumor suppressor/P53cellular tumor antigen p53/Phosphoprotein p53/transformation-related protein 53/tumor protein p53/Tumor suppressor p53

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt