Bcl-2 ELISA Kit

Produktnummer ABIN5026957

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Bcl-2.

Kurzübersicht für Bcl-2 ELISA Kit (ABIN5026957)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156-10 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Bcl-2 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Sensitivität: < 0.094 ng/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human Bcl-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human Bcl-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Bcl-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human Bcl-2 can be calculated using the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Andere Bezeichnung: Bcl -2 (B cell Lymphoma 2)

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling