AKT1 ELISA Kit

Produktnummer ABIN4885966

Produktdetails AKT1 ELISA Kit

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Bindungsspezifität: pSer473

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Phospho-AKT/GSKß/mTor ELISA Kit. This assay semi-quantitatively measures phophorylated AKT/GSK-beta/mTor in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes phosphorylated AKT (S473), GSK3? (S9), mTOR (S2448), and total AKT, GSK3?, mTOR in Human Cell Lysates.

Produktmerkmale:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Andere Bezeichnung: AKT (AKT1 Produkte)

Synonyme: AKT ELISA Kit, CWS6 ELISA Kit, PKB ELISA Kit, PKB-ALPHA ELISA Kit, PRKBA ELISA Kit, RAC ELISA Kit, RAC-ALPHA ELISA Kit, Akt ELISA Kit, Rac ELISA Kit, PKB/Akt ELISA Kit, PKBalpha ELISA Kit, AKT/PKB ELISA Kit, AKT1 ELISA Kit, Akt/PKB ELISA Kit, Akt1 ELISA Kit, CG4006 ELISA Kit, D-Akt ELISA Kit, DAKT1 ELISA Kit, DAKT1/PKB ELISA Kit, DAkt ELISA Kit, DAkt1 ELISA Kit, DPKB ELISA Kit, DRAC-PK ELISA Kit, DRAC-PK66 ELISA Kit, DRAC-PK85 ELISA Kit, Dakt ELISA Kit, Dakt1 ELISA Kit, Dmel\\CG4006 ELISA Kit, Dpkb ELISA Kit, PKB/AKT ELISA Kit, PKB/dAKT ELISA Kit, RacPK ELISA Kit, akt ELISA Kit, akt1 ELISA Kit, dAKT ELISA Kit, dAKT/dPKB ELISA Kit, dAKT1 ELISA Kit, dAkt ELISA Kit, dAkt/PKB ELISA Kit, dAkt1 ELISA Kit, dPKB ELISA Kit, dakt ELISA Kit, dakt1 ELISA Kit, l(3)04226 ELISA Kit, l(3)89Bq ELISA Kit, p-Akt ELISA Kit, pAkt ELISA Kit, ACT-5 ELISA Kit, akt-1 ELISA Kit, pkb ELISA Kit, v-akt ELISA Kit, v-akt1 ELISA Kit, xAct ELISA Kit, ATAKT1 ELISA Kit, F18A8.2 ELISA Kit, F18A8_2 ELISA Kit, K+ transporter 1 ELISA Kit, POTASSIUM TRANSPORTER ELISA Kit, AKT serine/threonine kinase 1 ELISA Kit, thymoma viral proto-oncogene 1 ELISA Kit, CG4006 gene product from transcript CG4006-RE ELISA Kit, actin beta ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 S homeolog ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 ELISA Kit, K+ transporter 1 ELISA Kit, Serine/threonine-protein kinase akt-1 ELISA Kit, AKT1 ELISA Kit, Akt1 ELISA Kit, ACTB ELISA Kit, akt1.S ELISA Kit, akt1 ELISA Kit, KT1 ELISA Kit, akt-1 ELISA Kit

Gen-ID: 391

UniProt: P31749

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt