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Nuclear extracts are prepared from WI-38 VA13 cells that have been stimulated with PMA/Ionomycin. TransAM assays are performed using 5 μg/well of extract with the c-Jun, c-Fos, FosB, Fra-1, Fra-2, JunB and JunD antibodies found in the TransAM AP-1 Family Kit. The TransAM assays are performed in the absence or presence of a 20X excess of competitor oligonucleotides, which contain either a wild-type or mutated AP-1 consensus binding site (Figure 1). The wild-type oligonucleotide prevents AP-1 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has a limited effect on AP-1 binding The relative activation of the family members may vary depending on the cell type tested and the manner in which it was stimulated.