PIK3CA Antikörper (AA 101-300)

Produktnummer ABIN968519

Der Maus Monoklonal Anti-PIK3CA-Antikörper wurde für WB und IF validiert. Er ist geeignet, PIK3CA in Proben von Human und Ratte zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für PIK3CA Antikörper (AA 101-300) (ABIN968519)

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PIK3CA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 19-PI3

Produktdetails anti-PIK3CA Antikörper

Bindungsspezifität: AA 101-300

Kreuzreaktivität: Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PI3-Kinase p110alpha aa. 101-300

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-PIK3CA Antikörper (ABIN968519)

Funaki, Katagiri, Kanda, Anai, Nawano, Ogihara, Inukai, Fukushima, Ono, Yazaki, Kikuchi, Oka, Asano: "p85/p110-type phosphatidylinositol kinase phosphorylates not only the D-3, but also the D-4 position of the inositol ring." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 22019-24, (1999) (PubMed).

Vicente-Manzanares, Rey, Jones, Sancho, Mellado, Rodriguez-Frade, del Pozo, Yáñez-Mó, de Ana, Martínez-A, Mérida, Sánchez-Madrid: "Involvement of phosphatidylinositol 3-kinase in stromal cell-derived factor-1 alpha-induced lymphocyte polarization and chemotaxis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 7, pp. 4001-12, (1999) (PubMed).

Katagiri, Asano, Ishihara, Inukai, Shibasaki, Kikuchi, Yazaki, Oka: "Overexpression of catalytic subunit p110alpha of phosphatidylinositol 3-kinase increases glucose transport activity with translocation of glucose transporters in 3T3-L1 adipocytes." in: The Journal of biological chemistry, Vol. 271, Issue 29, pp. 16987-90, (1996) (PubMed).

Hu, Klippel, Muslin, Fantl, Williams: "Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase." in: Science (New York, N.Y.), Vol. 268, Issue 5207, pp. 100-2, (1995) (PubMed).

Volinia, Hiles, Ormondroyd, Nizetic, Antonacci, Rocchi, Waterfield: "Molecular cloning, cDNA sequence, and chromosomal localization of the human phosphatidylinositol 3-kinase p110 alpha (PIK3CA) gene." in: Genomics, Vol. 24, Issue 3, pp. 472-7, (1995) (PubMed).

Details zu PIK3CA

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Andere Bezeichnung: PI3-Kinase p110 alpha

Hintergrund: Phosphatidylinositol 3 (PI3) -kinase participates in insulin-stimulated glucose uptake, PDGF-induced membrane ruffling, and G-protein receptor signaling. It exists as a heterodimer of 85 kDa (p85) and 110 kDa (p110) subunits. The p85 subunit associates with and serves as a substrate for activated growth factor receptor tyrosine kinases. p85 regulates the p110 catalytic subunit by acting as the link between PI3-kinase and the ligand-activated receptor. Four isoforms of p110 have been identified (alpha, beta, gamma, and delta). The p110alpha isoform contains an N-terminal region involved in p85 binding and a C-terminal kinase domain. p85/p110alpha-type PI kinase phosphorylates the D-3 and D-4 position of the inositol ring of PI, thereby producing PtdIns(3)P, PtdIns(3,4)P[2], PtdIns(3,4,5)P[3], PtdIns(4)P, and PtdIns(4,5) P[2]. During induction of chemotaxis by the chemokine SDF-1alpha, PI3-kinase regulates adhesion and ERM protein redistribution in the lymphocyte plasma membrane. In addition, PI3-kinases activate other signaling molecules, such as p70 S6 kinase and Akt/protein kinase B. Thus, p85/p110alpha-type PI kinase is a ubiquitously expressed kinase that is involved in a variety of cell signaling cascades.

Molekulargewicht: 110 kDa

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling