p53 Antikörper

Produktnummer ABIN967457

Der Maus Monoklonal Anti-p53-Antikörper wurde für WB, IP, IHC (f) und IHC (fro) validiert. Er ist geeignet, p53 in Proben von Human und Rind (Kuh) zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für p53 Antikörper (ABIN967457)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: DO

Produktdetails anti-p53 Antikörper

Marke: BD Pharmingen™

Kreuzreaktivität: Rind (Kuh)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human p53

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Applications include immunoprecipitation (1-2 µg/1x10^6 cells), western blot analysis (2 µg/ml), and immunohistochemistry of frozen and formalin-fixed paraffin-embedded tissue sections (5-20 µg/ml). Positive control cell lines include COS-7 SV40 transformed monkey kidney cells (ATCC CRL-1651), SK-BR-3 human breast carcinoma cells (ATCC HTB-30), and A431 human vulval carcinoma cells (ATCC CRL-1555). Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach or breast tissue.

Kommentare:

Related Products: ABIN967389, ABIN968533

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-p53 Antikörper (ABIN967457)

Friedrichs, Gluba, Eidtmann, Jonat: "Overexpression of p53 and prognosis in breast cancer." in: Cancer, Vol. 72, Issue 12, pp. 3641-7, (1994) (PubMed).

Xerri, Bouabdallah, Camerlo, Hassoun: "Expression of the p53 gene in Hodgkin's disease: dissociation between immunohistochemistry and clinicopathological data." in: Human pathology, Vol. 25, Issue 5, pp. 449-54, (1994) (PubMed).

Bartkova, Bartek, Vojtesek, Lukas, Rejthar, Kovarik, Millis, Lane, Barnes: "Immunochemical analysis of the p53 oncoprotein in matched primary and metastatic human tumours." in: European journal of cancer (Oxford, England : 1990), Vol. 29A, Issue 6, pp. 881-6, (1993) (PubMed).

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, (1992) (PubMed).

Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, (1991) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53

Hintergrund: The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and wild-type p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. DO-1 reacts with human wild-type and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-1 recognizes an epitope between amino acids 1 and 45 of all known forms of human p53. Human recombinant p53 protein was used as immunogen.

Molekulargewicht: 53 kDa

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt