AKT1 Antikörper (pSer472)

Produktnummer ABIN967296

Dieses Anti-AKT1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von AKT1 in WB und IP. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für AKT1 Antikörper (pSer472) (ABIN967296)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AKT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 104A282

Produktdetails anti-AKT1 Antikörper

Bindungsspezifität: pSer472

Marke: BD Pharmingen™

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Akt1 (pS473) Peptide

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-AKT1 Antikörper (ABIN967296)

Ferrigno, Silver: "Regulated nuclear localization of stress-responsive factors: how the nuclear trafficking of protein kinases and transcription factors contributes to cell survival." in: Oncogene, Vol. 18, Issue 45, pp. 6129-34, (1999) (PubMed).

Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, (1999) (PubMed).

Alessi, Andjelkovic, Caudwell, Cron, Morrice, Cohen, Hemmings: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, (1997) (PubMed).

Details zu AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Andere Bezeichnung: Akt

Hintergrund: Akt [also known as PKB (Protein kinase B) or RAC-PK (Related to the A and C kinases)] is a family of serine/threonine kinases that contains a pleckstrin-homology (PH) domain. PH domains play important roles in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (alpha), Akt2 (beta) and Akt3 (gamma)], they are thought to be regulated similarly. Akt is activated by insulin and growth factors by a mechanism involving phosphoinositide 3-OH kinase. Phosphoinositide 3-OH kinases products bind to the pleckstrin homology domain resulting in translocation of Akt to the plasma membrane and activation of Akt to phospho-Akt by upstream kinases. Akt is phosphorylated within the activation loop at threonine 308 and the C-terminus at serine 473. Phospho-Akt promotes cell survival by inhibiting apoptosis. Specifically, phospho-Akt1 has been shown to phosphorylate Bad, a member of the Bcl-2 family that promotes cell death. This phosphorylation results in the inactivation of the proapoptotic function of Bad. The Akt molecule is thus considered to link extracellular survival signals (growth factors) with the apoptotic machinery (Bad). Akt is also a key mediator of the metabolic effects of insulin. Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors. The 104A282 antibody recognizes Akt1 phosphorylated at S473 and Akt3 phosphorylated at S472. This antibody may also recognize Akt2 when phosphorylated at S474.
Synonyms: Akt1, Akt2, Akt3, PKBalpha, PKBbeta, PKBgamma, RAC-PKalpha, RAC-PKbeta, RAC-PKgamma, STK-2

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt