eIF4EBP1 Antikörper

Produktnummer ABIN965485

Dieser Monoklonal Antikörper detektiert spezifisch eIF4EBP1 in IHC und ELISA. Es zeigt Reaktivität gegenüber Proben von Human. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für eIF4EBP1 Antikörper (ABIN965485)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reaktivität: Human

Wirt: Bitte anfragen

Klonalität: Monoklonal

Konjugat: Dieser eIF4EBP1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA

Produktdetails anti-eIF4EBP1 Antikörper

Spezifität: Ni-NTA purified truncated recombinant 4EBP1 expressed in E. Coli strain BL21 (DE3)

Aufreinigung: Antibodies are purified by protein A affinity chromatography

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: IHC(P): 1: 500- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Referenzen für anti-eIF4EBP1 Antikörper (ABIN965485)

Fadden, Haystead, Lawrence: "Identification of phosphorylation sites in the translational regulator, PHAS-I, that are controlled by insulin and rapamycin in rat adipocytes." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10240-7, (1997) (PubMed).

Pause, Belsham, Gingras, Donzé, Lin, Lawrence, Sonenberg: "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function." in: Nature, Vol. 371, Issue 6500, pp. 762-7, (1994) (PubMed).

Details zu eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Andere Bezeichnung: 4E-BP1

Hintergrund: 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Gen-ID: 1978

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, BCR Signaling