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AKT1 Antikörper (C-Term)

This anti-AKT1 antibody (ABIN94783) is a Rabbit Polyclonal antibody detecting AKT1 in WB, ELISA, IHC, IF, FACS, FM. Suitable for Human, Mouse, Rat, Chicken.
Rockland
Produktnummer ABIN94783
Hersteller Produkt- Nr.: 100-401-401

Quick Overview for AKT1 Antikörper (C-Term) (ABIN94783)

Target

Alle AKT1 Antikörper anzeigen
AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reaktivität

  • 488
  • 312
  • 248
  • 53
  • 51
  • 45
  • 33
  • 18
  • 12
  • 11
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte, Huhn

Wirt

  • 405
  • 104
  • 7
  • 2
  • 1
  • 1
Kaninchen

Klonalität

  • 359
  • 160
Polyklonal

Konjugat

  • 305
  • 29
  • 20
  • 20
  • 19
  • 13
  • 10
  • 10
  • 8
  • 8
  • 8
  • 8
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Dieser AKT1 Antikörper ist unkonjugiert

Applikation

  • 405
  • 233
  • 171
  • 89
  • 77
  • 69
  • 60
  • 50
  • 38
  • 35
  • 31
  • 18
  • 15
  • 9
  • 9
  • 8
  • 6
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM)
  • Bindungsspezifität

    • 89
    • 44
    • 35
    • 33
    • 27
    • 23
    • 19
    • 18
    • 16
    • 12
    • 11
    • 10
    • 9
    • 8
    • 8
    • 8
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    C-Term

    Hersteller Produkt- Nr.

    100-401-401

    Hersteller

    Rockland

    Verwendungszweck

    AKT Antibody

    Kreuzreaktivität (Details)

    Pan Anti-AKT Antibody reacts with the AKT from human tissues.

    Produktmerkmale

    Synonyms: rabbit anti-AKT antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT Serine/Threonine Kinase 1, AKT 1 Antibody, AKT-1 Antibody

    Aufreinigung

    This product was prepared from monospecific antiserum by a delipidation and defibrination.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. A BLAST analysis was used to suggest reactivity with this protein from rat, mouse, and chicken based on 100% homology for the immunogen sequence.

    Immunogen Type: Conjugated Peptide

  • Applikationshinweise

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: 1:500 - 1:2,000

    Application Note: Anti-AKT Antibody has been tested in Western Blot, Immunohistochemistry (Formalin-fixed paraffin-embedded sections), and Immunofluorescence (paraformaldehyde-fixed primary cardiomyocyte cultures). Expect a band at ~55.7 kDa in 3T3 whole cell lysate or other appropriate cell lysates or tissues in western blot. Although not tested, this antibody would be useful in flow cytometry. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:2,000 - 1:10,000

    IF Microscopy Dilution: 1:100 - 1:1,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store Anti-Akt antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Sato, Uzu, Kashiba, Suzuki, Fujiwara, Okuzawa, Ueno: "Sodium butyrate enhances the growth inhibitory effect of sunitinib in human renal cell carcinoma cells." in: Oncology letters, Vol. 14, Issue 1, pp. 937-943, (2017) (PubMed).

    Takeuchi, Ryo, Komitsu, Mikuni-Takagaki, Fukui, Takagi, Shiraishi, Morishita, Yamazaki, Kumagai, Aoki, Saito: "Low-intensity pulsed ultrasound activates the phosphatidylinositol 3 kinase/Akt pathway and stimulates the growth of chondrocytes in three-dimensional cultures: a basic science study." in: Arthritis research & therapy, Vol. 10, Issue 4, pp. R77, (2008) (PubMed).

    Matsuda, Takano, Kageyama, Hatakeyama, Shakunaga, Kitajima, Yamazaki, Hattori: "Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock." in: Cardiovascular research, Vol. 76, Issue 1, pp. 132-40, (2007) (PubMed).

    Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, (2001) (PubMed).

  • Target

    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

    Andere Bezeichnung

    AKT1

    Hintergrund

    Background: AKT Antibody detects AKT which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction, VEGF Signaling, and apoptosis research.

    Gen-ID

    207, 62241011

    UniProt

    P31749

    Pathways

    PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt
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