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p53 Antikörper

Der Maus Monoklonal Anti-p53-Antikörper wurde für WB, ELISA, FACS, IHC (p), IP und ICC validiert. Er ist geeignet, p53 in Proben von Human und Non-Human Primate zu detektieren. Es sind 4+ Publikationen verfügbar.
Produktnummer ABIN94448

Kurzübersicht für p53 Antikörper (ABIN94448)

Target

Alle p53 (TP53) Antikörper anzeigen
p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität

  • 968
  • 280
  • 232
  • 143
  • 75
  • 74
  • 60
  • 40
  • 10
  • 9
  • 9
  • 6
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Non-Human Primate

Wirt

  • 671
  • 323
  • 5
  • 2
  • 2
Maus

Klonalität

  • 576
  • 426
  • 1
Monoklonal

Konjugat

  • 527
  • 51
  • 32
  • 20
  • 19
  • 18
  • 17
  • 17
  • 17
  • 17
  • 17
  • 17
  • 14
  • 14
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  • 14
  • 12
  • 12
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  • 11
  • 11
  • 11
  • 10
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Dieser p53 Antikörper ist unkonjugiert

Applikation

  • 732
  • 323
  • 286
  • 240
  • 212
  • 141
  • 132
  • 129
  • 122
  • 119
  • 79
  • 64
  • 35
  • 20
  • 17
  • 12
  • 10
  • 9
  • 8
  • 6
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon

BP53-12
  • Verwendungszweck

    Anti-p53 Purified

    Spezifität

    The antibody BP53-12 recognizes a defined epitope (aa 16-25) on human p53, a 50 kDa intracellular tumour suppressor found in increased amounts in a wide variety of transformed cells, it is frequently mutated or inactivated in many types of cancer.

    Kreuzreaktivität (Details)

    Human, Non-Human Primates

    Aufreinigung

    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

    Reinheit

    > 95 % (by SDS-PAGE)

    Immunogen

    Bacterially expressed full-length wild-type p53

    Isotyp

    IgG2a
  • Applikationshinweise

    Western blotting: Recommended dilution: 1-2 μg/mL, overnight at 4 °C, positive control: RAMOS human lymphoma cell line, non-reducing conditions. SDS-PAGE (12 % separating gel).
    Immunohistochemistry: Recommended dilution: 5-10 μg/mL.
    Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Do not freeze.
  • Yoshitake, Aoyagi, Fujiwara: "Creation of a novel telomere-cutting endonuclease based on the EN domain of telomere-specific non-long terminal repeat retrotransposon, TRAS1." in: Mobile DNA, Vol. 1, Issue 1, pp. 13, (2010) (PubMed).

    Dolezalová, Vojt?sek, Kovarík: "Epitope analysis of the human p53 tumour suppressor protein." in: Folia biologica, Vol. 43, Issue 1, pp. 49-51, (1997) (PubMed).

    Bártek, Bártková, Vojt?sek, Stasková, Lukás, Rejthar, Kovarík, Midgley, Gannon, Lane: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." in: Oncogene, Vol. 6, Issue 9, pp. 1699-703, (1991) (PubMed).

    Bártková, Bártek, Lukás, Vojt?sek, Stasková, Rejthar, Kovarík, Midgley, Lane: "p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 2, pp. 196-202, (1991) (PubMed).

  • Target

    p53 (TP53) (Tumor Protein P53 (TP53))

    Andere Bezeichnung

    p53

    Hintergrund

    Tumor protein p53,The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.,BCC7, TRP53, TP53, LFS1

    Gen-ID

    7157

    UniProt

    P04637

    Pathways

    p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt
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