MAP3K1 Antikörper (AA 1211-1310)

Produktnummer ABIN7865694

Der Maus Monoklonal anti-MAP3K1 Antikörper wird verwendet zum Nachweis von MAP3K1 in Proben von Human. Er wurde validiert für WB, ELISA, IHC, IF, ICC und FACS.

Kurzübersicht für MAP3K1 Antikörper (AA 1211-1310) (ABIN7865694)

Target: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAP3K1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: EBS-T-008

Produktdetails anti-MAP3K1 Antikörper

Bindungsspezifität: AA 1211-1310

Verwendungszweck: anti-MAP3K1 mouse monoclonal, EBS-T-008, purified

Aufreinigung: affinity chromatography

Immunogen: partial recombinant MAP3K1 (aa1211-1310)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

ICC/IF_dilution: assay dependent

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:100 - 1:200 (0,5-1,0 μg/mL, microwave treatment in 10 mM Tris with 1 mM EDTA pH 9.0 recommended)

WB_dilution: 1:100-1:200 (0.5-1 μg/mL)

IHC-frozen_dilution: 1:100 - 1:200 (0,5-1,0 μg/mL )

elisa_dilution: assay dependent

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Details zu MAP3K1

Target: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Andere Bezeichnung: MAP3K1

Hintergrund: MAPKs are involved in directing cellular responses to a diverse array of potentially harmful stimuli, such as mitogens, osmotic stress, heat shock, proinflammatory cytokines, but also growth factors (mammals). Possibly located exclusively in the cell nucleus, they regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. In order to become active, they require usually multiple phosphorylation events in their activation loops, including phosphorylation by MAP2 kinases (Ste-7 kinases), which in turn are phosphorylated by the MAP3 kinase family, of which many are located at the cell membrane. Thus through this pathway, stimuli can effectively be conveyed from the cell membrane to the nucleus. Inactivation of MAPKs takes place by several phosphorylases, including dedicated phophorylases. Positive control: A431, HeLa or HL-60 cells or liver tissue.

Pathways: MAPK Signalweg, Interferon-gamma Pathway, Caspase Kaskade in der Apoptose, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades