Insulin Antikörper (CF®488A)

Produktnummer ABIN7655303

Der Maus Monoklonal Anti-Insulin-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, Insulin in Proben von Human zu detektieren.

Kurzübersicht für Insulin Antikörper (CF®488A) (ABIN7655303)

Target: Insulin (INS)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Insulin Antikörper ist konjugiert mit CF®488A

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: K36aC10

Produktdetails anti-Insulin Antikörper (CF®488A)

Verwendungszweck: Insulin / IRDN (beta-Cell & Insulinoma Marker)(K36aC10), CF488A conjugate

Produktmerkmale: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human insulin

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: MIA PaCa-2 cells. Pancreas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Insulin

Target: Insulin (INS)

Andere Bezeichnung: Insulin

Hintergrund:

Synonyms: IDDM2, ILPR, IRDN, MODY10, Proinsulin

Gene Symbol: INS

Tissue Expression: Pancreatic beta cells

Molekulargewicht: 6 kDa

Gen-ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation