AKT1 Antikörper (pSer473) (PE-Cy5)

Produktnummer ABIN729310

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch AKT1 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Huhn, Rind (Kuh), Hund und Schwein.

Kurzübersicht für AKT1 Antikörper (pSer473) (PE-Cy5) (ABIN729310)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh), Hund, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKT1 Antikörper ist konjugiert mit PE-Cy5

Applikation: Western Blotting (WB)

Produktdetails anti-AKT1 Antikörper (PE-Cy5)

Bindungsspezifität: pSer473

Kreuzreaktivität: Huhn, Rind (Kuh), Fisch, Human, Maus, Kaninchen, Ratte

Homologie: Dog,Cow,Sheep

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FCM(1:100-500)
Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Andere Bezeichnung: AKT

Hintergrund:

Synonyms: Akt, RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, RAC-PK-alpha, Akt1

Background: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Molekulargewicht: 56kDa

Gen-ID: 24185

UniProt: P47196

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt