p53 Antikörper (acLys292)

Produktnummer ABIN7565900

Der Kaninchen Polyklonal anti-p53 Antikörper wird verwendet zum Nachweis von p53 in Proben von Human. Er wurde validiert für WB, ELISA und DB.

Kurzübersicht für p53 Antikörper (acLys292) (ABIN7565900)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB)

Produktdetails anti-p53 Antikörper

Bindungsspezifität: acLys292

Verwendungszweck: p53 K292 Ac Antibody

Kreuzreaktivität (Details): Anti-p53 K292 Ac antibody is directed against human p53 protein.

Produktmerkmale: Synonyms: rabbit anti-p53 Ac-Lys292 antibody, rabbit anti-p53 K292ac antibody, cellular tumor antigen p53, tumor suppressor p53, phosphoprotein p53, Antigen NY-CO-13, TP53

Aufreinigung: p53 K292 Ac Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-p53 K292 Ac antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 292 of human p53.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: p53 K292 Ac Antibody has been tested by dot blot and western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~43.6 kDa in size corresponding to p53 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:5000 - 1:10000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: TP53

Hintergrund: Background: TP53 (tumor suppressor gene p53) is one of the most well-studied genes that suppresses tumor formation and renders protection against DNA damage by inducing cell cycle arrest, DNA repair, or apoptosis. TP53 signaling is triggered through numerous cellular events ranging from DNA damage to hypoxia, stress and a plethora of other causes. Upon activation, p53 acts as zinc-containing transcriptional regulator and initiates a cascade of events that determines the cellular outcome including cell cycle arrest, apoptosis, senescence, DNA repair, development, differentiation and tissue homeostasis. Cell cycle arrest is induced by p53 via trans-activating genes such as p21 (CDK-inhibitor 1, cyclin dependent kinase) and others. Interestingly, p53 itself is capable of triggering cellular responses (survival or induced cell death) as well. Mutations or deletions in the TP53 gene are present in nearly 50 % of human cancers, and primarily results in impaired tumor suppressor function. Anti-p53 (ac Lys292) antibody is ideal for researchers interested in developmental biology, cell growth and cancer research.

Gen-ID: 7157

NCBI Accession: NP_000537

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt