Bcl-2 Antikörper (AA 50-100)
Kurzübersicht für Bcl-2 Antikörper (AA 50-100) (ABIN7454495)
Target
Alle Bcl-2 (BCL2) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 50-100
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Verwendungszweck
- Rabbit anti-Bcl-2 Antibody, Affinity Purified
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Aufreinigung
- Affinity Purified
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Immunogen
- Between AA 50 and 100
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Isotyp
- IgG
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Applikationshinweise
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IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IP: 2 - 10 μg/mg lysate
WB: Not recommended. A303-675A has not performed satisfactorily when used for WB of Bcl-2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Bcl-2.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1000 μg/mL
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Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Haltbarkeit
- 12 months
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- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
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Andere Bezeichnung
- Bcl-2
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Hintergrund
- Background: B-cell CLL/lymphoma 2 (Bcl-2) is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl-2, such as in the case of translocation of Bcl-2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma [taken from NCBI Entrez Gene (Gene ID: 596)].
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Gen-ID
- 596
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NCBI Accession
- NP_000624
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UniProt
- P10415
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Pathways
- MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
Target
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