Phospholipase C gamma 2 Antikörper

Produktnummer ABIN7223186

Der Kaninchen Polyklonal anti-Phospholipase C gamma 2 Antikörper wird verwendet zum Nachweis von Phospholipase C gamma 2 in Proben von Human, Ratte und Maus. Er wurde validiert für WB, IHC, ELISA und IF.

Kurzübersicht für Phospholipase C gamma 2 Antikörper (ABIN7223186)

Target: Phospholipase C gamma 2 (PLCG2)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Phospholipase C gamma 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-Phospholipase C gamma 2 Antikörper

Verwendungszweck: Rabbit Anti-PLC γ2 Polyclonal Antibody

Spezifität: PLC γ2 Polyclonal Antibody detects endogenous levels of PLC γ2 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PLC gamma2 around the non-phosphorylation site of Y1217

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Andere Bezeichnung: PLC gamma2

Hintergrund: PLCG2, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, PLC-IV, Phospholipase C-gamma-2, PLC-gamma-2The protein encoded by PLCG2 (phospholipase C gamma 2) is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in PLCG2 have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

Molekulargewicht: 147kD

Gen-ID: 5336

UniProt: P16885

Pathways: RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling