p53 Antikörper (pSer15)

Produktnummer ABIN7138434

Produktdetails anti-p53 Antikörper

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Bindungsspezifität: pSer15

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Kreuzreaktivität: Human

Aufreinigung: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 15 (P-L-S(p)-Q-E) derived from Human p53.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: TP53 (TP53 Produkte)

Hintergrund:

Background:

Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Lin T, et al. (2005) Nat Cell Biol, 7(2): 165-71.
Vega FM, et al. (2004) Mol Cell Biol, 24(23): 10366-80.
Li J, et al. (2004) J Biol Chem, 279(40): 41275-9.
Wang J, et al. (2004) J Biol Chem, 279(38): 39584-92.

Aliases: Antigen NY-CO-13 antibody, BCC7 antibody, Cellular tumor antigen p53 antibody, FLJ92943 antibody, LFS1 antibody, Mutant tumor protein 53 antibody, p53 antibody, p53 tumor suppressor antibody, P53_HUMAN antibody, Phosphoprotein p53 antibody, Tp53 antibody, Transformation related protein 53 antibody, TRP53 antibody, tumor antigen p55 antibody, Tumor protein 53 antibody, Tumor protein p53 antibody, Tumor suppressor p53 antibody

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt