Rekombinanter AKT1 Antikörper (AA 85-189)

Produktnummer ABIN7126340

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch AKT1 in WB, ELISA und Coat. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter AKT1 Antikörper (AA 85-189) (ABIN7126340)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser AKT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Coating (Coat)

Produktdetails anti-AKT1 Antikörper

Bindungsspezifität: AA 85-189

Spezifität: Recognizes a protein of 62 kDa, which is identified as AKT1The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), ELISA (For coating, order antibody without BSA),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Andere Bezeichnung: AKT1

Hintergrund: Oncogene AKT1, PKB, PKB-ALPHA, PRKBA, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC Alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha,AKT1 (Prognostic Marker for Neuroendocrine Tumors)
Cellular localisation: Nuclear, Cytoplasmic and Cell Surface

Molekulargewicht: 62kDa

Gen-ID: 207, 525622

UniProt: P31749

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt