eIF4EBP1 Antikörper (C-Term)

Produktnummer ABIN6990540

Dieses Anti-eIF4EBP1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von eIF4EBP1 in WB, ELISA, IF und ICC. Geeignet für Human und Maus.

Kurzübersicht für eIF4EBP1 Antikörper (C-Term) (ABIN6990540)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser eIF4EBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-eIF4EBP1 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: 4E-BP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: 4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1. The immunogen is located within the last 50 amino acids of 4E-BP1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 - 10 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 4E-BP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: 4E-BP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Andere Bezeichnung: 4E-BP1

Hintergrund: 4E-BP1 Antibody: The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferationl, at least in part by inhibiting the activity of TOR towards 4E-BP1.

Gen-ID: 1978

NCBI Accession: NP_004086

UniProt: Q13541

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, BCR Signaling