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p53 Antikörper

Dieses Anti-p53-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von p53 in WB, IHC, IF, FACS und StM. Geeignet für Human.
Produktnummer ABIN6940788

Kurzübersicht für p53 Antikörper (ABIN6940788)

Target

Alle p53 (TP53) Antikörper anzeigen
p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität

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Human

Wirt

  • 671
  • 323
  • 5
  • 2
  • 2
Maus

Klonalität

  • 576
  • 426
  • 1
Monoklonal

Konjugat

  • 527
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Dieser p53 Antikörper ist unkonjugiert

Applikation

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon

DO-1
  • Spezifität

    Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 20-25) of p53. Monoclonal antibody PAb1801 does not block the binding of DO-7 MAb to p53 in an ELISA test. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

    Keine Kreuzreaktivität

    Affe, Ratte (Rattus)

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Recombinant human wild type p53 protein expressed in E. coli.

    Isotyp

    IgG2a kappa
  • Applikationshinweise

    Positive Control: MDA-MB-231 cells. Breast or Colon carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    p53 (TP53) (Tumor Protein P53 (TP53))

    Andere Bezeichnung

    TP53

    Molekulargewicht

    53kDa

    Gen-ID

    7157

    UniProt

    P04637

    Pathways

    p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt
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