Insulin Antikörper

Produktnummer ABIN6939789

Der Maus Monoklonal Anti-Insulin-Antikörper wurde für IHC, IF, IHC (p), FACS, ISt und StM validiert. Er ist geeignet, Insulin in Proben von Human, Schwein, Maus und Rind (Kuh) zu detektieren.

Kurzübersicht für Insulin Antikörper (ABIN6939789)

Target: Insulin (INS)

Reaktivität: Human, Schwein, Maus, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Insulin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)

Klon: 2D11-H5-same as INS05

Produktdetails anti-Insulin Antikörper

Spezifität: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Aufreinigung: Purified by Protein A/G

Immunogen: Purified pig insulin, conjugated to KLH

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MIA PaCa-2 cells. Pancreas.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 minutes at RT)(No special pretreatment is required for staining of formalin/paraffin tissues.)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Insulin

Target: Insulin (INS)

Andere Bezeichnung: INS

Molekulargewicht: 6kDa

Gen-ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation