Insulin Antikörper

Produktnummer ABIN6937564

Dieses Anti-Insulin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Insulin in IF, IHC (p), FACS, IHC (f) und ISt. Geeignet für Schwein.

Kurzübersicht für Insulin Antikörper (ABIN6937564)

Target: Insulin (INS)

Reaktivität: Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Insulin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Klon: SPM139

Produktdetails anti-Insulin Antikörper

Spezifität: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Kreuzreaktivität (Details): Human. Cow. Pig. Does not react with Mouse or Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Full length (1-84 amino acid) Purified pig insulin, conjugated to KLH

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(No special pretreatment is required for staining of formalin/paraffin tissues.)Optimal dilution for a specific application should be determined.

Positive_Control: Pancreas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Insulin

Target: Insulin (INS)

Andere Bezeichnung: INS

Hintergrund: IDDM2, ILPR, IRDN, MODY10, Proinsulin,Insulin (beta-Cell & Insulinoma Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 6kDa

Gen-ID: 3630, 272259

UniProt: P01308, P01317

Pathways: NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation