p53 Antikörper (AA 301-393)

Produktnummer ABIN677408

Der Kaninchen Polyklonal Anti-p53-Antikörper wurde für WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc) und IHC (fro) validiert. Er ist geeignet, p53 in Proben von Human, Maus, Ratte und Ziege zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für p53 Antikörper (AA 301-393) (ABIN677408)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human, Maus, Ratte, Ziege

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-p53 Antikörper

Bindungsspezifität: AA 301-393

Kreuzreaktivität: Ziege, Human, Maus, Ratte

Homologie: Dog,Sheep,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human P53

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-p53 Antikörper (ABIN677408)

Zhang, Zhang, Liu, Huang, Zhang, Yi, Yao, Hu: "Therapeutic efficacy of paclitaxel and carboplatin via arterial or venous perfusion in rabbits with VX-2 tongue cancer." in: International journal of clinical and experimental medicine, Vol. 8, Issue 4, pp. 4979-88, (2015) (PubMed).

Chen, Wang, Wang, Huang, Zhang: "STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7." in: Cancer cell international, Vol. 15, pp. 111, (2015) (PubMed).

Yang, Hui, Yuqin, Jie, Shuai, Tiezhu, Wei: "Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 563-566, (2014) (PubMed).

Yang, Gu, Zheng, Li, Lian, Miao, Jiang, Wei: "RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11865-74, (2011) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53

Hintergrund:

Synonyms: P53, BCC7, LFS1, TRP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53

Background: Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis, the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Gen-ID: 7157

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt