AKT1 Antikörper (AA 121-170)

Produktnummer ABIN6736701

Produktdetails anti-AKT1 Antikörper

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Bindungsspezifität: AA 121-170

Reaktivität: Human, Maus, Ratte, Huhn, Rind (Kuh), Hund, Affe, Schaf, Ziege, Xenopus laevis, Pferd, Hamster, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human AKT1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Chicken, Xenopus (100%) Pig (93%) Sheep, Bovine, Goat (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa121-170 of human AKT1 (P31749, NP_005154). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (100%), Pig, Lizard (93%), Sheep, Goat, Bovine (86%), Baboon, Elephant, Rabbit, Salmon (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Andere Bezeichnung: AKT1 / AKT (AKT1 Produkte)

Synonyme: AKT antikoerper, CWS6 antikoerper, PKB antikoerper, PKB-ALPHA antikoerper, PRKBA antikoerper, RAC antikoerper, RAC-ALPHA antikoerper, Akt antikoerper, Rac antikoerper, PKB/Akt antikoerper, PKBalpha antikoerper, AKT/PKB antikoerper, AKT1 antikoerper, Akt/PKB antikoerper, Akt1 antikoerper, CG4006 antikoerper, D-Akt antikoerper, DAKT1 antikoerper, DAKT1/PKB antikoerper, DAkt antikoerper, DAkt1 antikoerper, DPKB antikoerper, DRAC-PK antikoerper, DRAC-PK66 antikoerper, DRAC-PK85 antikoerper, Dakt antikoerper, Dakt1 antikoerper, Dmel\\CG4006 antikoerper, Dpkb antikoerper, PKB/AKT antikoerper, PKB/dAKT antikoerper, RacPK antikoerper, akt antikoerper, akt1 antikoerper, dAKT antikoerper, dAKT/dPKB antikoerper, dAKT1 antikoerper, dAkt antikoerper, dAkt/PKB antikoerper, dAkt1 antikoerper, dPKB antikoerper, dakt antikoerper, dakt1 antikoerper, l(3)04226 antikoerper, l(3)89Bq antikoerper, p-Akt antikoerper, pAkt antikoerper, ACT-5 antikoerper, akt-1 antikoerper, pkb antikoerper, v-akt antikoerper, v-akt1 antikoerper, xAct antikoerper, ATAKT1 antikoerper, F18A8.2 antikoerper, F18A8_2 antikoerper, K+ transporter 1 antikoerper, POTASSIUM TRANSPORTER antikoerper, AKT serine/threonine kinase 1 antikoerper, thymoma viral proto-oncogene 1 antikoerper, CG4006 gene product from transcript CG4006-RE antikoerper, actin beta antikoerper, v-akt murine thymoma viral oncogene homolog 1 S homeolog antikoerper, v-akt murine thymoma viral oncogene homolog 1 antikoerper, K+ transporter 1 antikoerper, Serine/threonine-protein kinase akt-1 antikoerper, AKT1 antikoerper, Akt1 antikoerper, ACTB antikoerper, akt1.S antikoerper, akt1 antikoerper, KT1 antikoerper, akt-1 antikoerper

Hintergrund: Name/Gene ID: AKT1
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA

Gen-ID: 207

NCBI Accession: NP_005154

UniProt: P31749

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt