AKT1 Antikörper (Internal Region)

Produktnummer ABIN6391337

Der Ziege Polyklonal anti-AKT1 Antikörper wird verwendet zum Nachweis von AKT1 in Proben von Human und Maus. Er wurde validiert für WB und ELISA.

Kurzübersicht für AKT1 Antikörper (Internal Region) (ABIN6391337)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AKT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Güteklasse: Verified

Produktdetails anti-AKT1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: AKT1

Sequenz: EVSLAKPKHR

Spezifität: Variants one, two and three encode the same protein (NP_005154, NP_001014432, NP_001014431). No cross-reactivity expected with AKT2 or AKT3.

Kreuzreaktivität: Rind (Kuh), Human, Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 60 kDa band observed in MCF7 and approx. 60-65 kDa in NIH3T3 cell lysates (calculated MW of 55.7 kDa according to Human NP_005154.2 and Mouse NP_033782.1). This molecular weight is routinely observed by other sources. Recommended conce

Peptide ELISA: antibody detection limit dilution 1:16000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Andere Bezeichnung: AKT1

Hintergrund: AKT1, v-akt murine thymoma viral oncogene homolog 1 , HGNC:391, MGC99656, PKB, PRKBA, RAC, RAC-ALPHA , RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, protein kinase B, rac protein kinase alpha

Gen-ID: 207

NCBI Accession: NP_005154, NP_001014432, NP_001014431

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt