Bcl-2 Antikörper (AA 41-54)

Produktnummer ABIN6294524

Der Maus Monoklonal anti-Bcl-2 Antikörper wird verwendet zum Nachweis von Bcl-2 in Proben von Human. Er wurde validiert für WB, IHC, IF, FACS und IP.

Kurzübersicht für Bcl-2 Antikörper (AA 41-54) (ABIN6294524)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Bcl-2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Produktdetails anti-Bcl-2 Antikörper

Bindungsspezifität: AA 41-54

Verwendungszweck: Mouse anti-Human Bcl2 Antibody Cocktail [Sodium Azide Free]

Spezifität: Cytoplasmic, membrane

Aufreinigung: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG) of the human protein was used as the immunogen for both antibodies in this Bcl2 antibody cocktail.

Immunogen: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG) of the human protein was used as the immunogen for both antibodies in this Bcl2 antibody cocktail.

Anwendungsinformationen

Applikationshinweise: Flow: 0.5-1 μg/million cells
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Andere Bezeichnung: Apoptosis regulator Bcl-2

Hintergrund:

Target Description: B-cell lymphoma 2 is an oncogene that regulates apoptosis, having both inducing and inhibiting functions. In cancer cells, Bcl2 binds pro-apoptotic proteins, preventing programmed cell death from occurring. Overexpression is seen in many cancers including lung, breast, prostate and lymphomas. In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies.

Gene Symbol: BCL2

Gen-ID: 596

UniProt: P10415

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling