Bcl-2 Antikörper (AA 41-54)

Produktnummer ABIN6294520

Dieser Maus Monoklonal Antikörper detektiert spezifisch Bcl-2 in WB, IHC, IF, FACS, ELISA und Coat. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Bcl-2 Antikörper (AA 41-54) (ABIN6294520)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Bcl-2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Coating (Coat)

Produktdetails anti-Bcl-2 Antikörper

Bindungsspezifität: AA 41-54

Verwendungszweck: Mouse anti-Human Bcl-2 Antibody [Sodium Azide Free]

Spezifität: Cytoplasmic, membrane

Aufreinigung: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.

Immunogen: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.

Anwendungsinformationen

Applikationshinweise: ELISA: order BSA-free format for coating
FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Andere Bezeichnung: Apoptosis regulator Bcl-2

Hintergrund:

Target Description: This antibody recognizes a protein of 25-26 kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.

Gene Symbol: BCL2

Gen-ID: 596

UniProt: P10415

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling