IL-1 beta Antikörper

Produktnummer ABIN616079

Der Kaninchen Polyklonal anti-IL-1 beta Antikörper wird verwendet zum Nachweis von IL-1 beta in Proben von Maus und Ratte. Er wurde validiert für WB, IP, IF, IHC (p), Neut, EIA, IHC (fro) und RIA.

Kurzübersicht für IL-1 beta Antikörper (ABIN616079)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-1 beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Produktdetails anti-IL-1 beta Antikörper

Spezifität: This antibody is primarily directed against mature, 17,000 MW Mouse IL1-beta and is useful in determining its presence in various assays. The antibody does not recognize Human IL1-beta or Mouse IL1-alpha. In ELISA formats and other immunoreactive assays, reactivity occurs with Rat IL1-beta. This antibody will recognize 10 % of the non-denatured (native) precursor 31,000 MW Mouse IL1-beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in Immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1-beta precursor molecule. Denatured 31,000 precursor IL1-beta will be recognized by this antibody.

Aufreinigung: DEAE Fractionation

Immunogen: Recombinant Mouse IL-1 beta produced in E. coli Genename: IL1b Remarks: The MW of the recombinant Mouse IL-1 beta is 17 kDa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.1 mL of deionized water (or equivalent)

Konzentration: 1.0 mg/mL (UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, None, None

Konservierungsmittel: Azide free

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C or -20 °C. Following reconstitution store at -20 °C.

Details zu IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: Interleukin-1 beta / IL-1B

Hintergrund: IL-1 beta (also known as Interleukin-1 beta, IL1-beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2

Gen-ID: 16176

NCBI Accession: NP_032387

UniProt: P10749

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome