Insulin Antikörper (CF®594)

Produktnummer ABIN6157353

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Insulin in IF, FACS und IHC (f). Er zeigt eine Reaktivität gegenüber Human, Schwein, Maus und Rind (Kuh).

Kurzübersicht für Insulin Antikörper (CF®594) (ABIN6157353)

Target: Insulin (INS)

Reaktivität: Human, Schwein, Maus, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Insulin Antikörper ist konjugiert mit CF®594

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 2D11-H5

Produktdetails anti-Insulin Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-Insulin (2D11-H5), CF594 Conjugate

Produktmerkmale: This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified pig insulin, conjugated to KLH

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

MIA PaCa-2 cells or Pancreas

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Insulin

Target: Insulin (INS)

Andere Bezeichnung: Insulin

Hintergrund: IDDM2, ILPR, IRDN, MODY10, Proinsulin

Molekulargewicht: 6 kDa

Gen-ID: 3630, 272259

UniProt: P01308

Pathways: NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation