AKT1 Antikörper (C-Term)

Produktnummer ABIN615022

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch AKT1 in WB, IF, IHC (p), IP und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte und Huhn.

Kurzübersicht für AKT1 Antikörper (C-Term) (ABIN615022)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Produktdetails anti-AKT1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A

Spezifität: Recognizes AKT (AKT1/AKT2/AKT3). The sequence used to generate this antibody, has a high degree of similarity to regions found in AKT1, AKT2 and AKT3, and thus may cross react with all of these proteins.

Kreuzreaktivität (Details): Species reactivity (expected):Chicken.
Species reactivity (tested):Human, Mouse and Rat.

Aufreinigung: Delipidation and Defibrination

Immunogen: Synthetic peptide -KLH conjugated corresponding to the C-terminus (460-480) of Human, Rat, Mouse and Chicken AKT proteins conjugated to KLH using maleimide.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Dilute only prior to immediate use.

Details zu AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Andere Bezeichnung: AKT1 / PKB

Hintergrund: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis and (ii) promotion of proliferation.Synonyms: AKT-2, AKT-3 Review 010, Akt-1, C-AKT, PKB gamma, PKBG, Protein kinase B, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma

Gen-ID: 207

NCBI Accession: NP_001014431

UniProt: P31749

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt