IL12 Antikörper

Produktnummer ABIN596134

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch IL12 in ELISA, WB und Neut. Er zeigt eine Reaktivität gegenüber Hund.

Kurzübersicht für IL12 Antikörper (ABIN596134)

Target: IL12 (Interleukin 12 (IL12))

Reaktivität: Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL12 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Neutralization (Neut)

Produktdetails anti-IL12 Antikörper

Spezifität: This antibody recognizes both the p35 and p40 sub-units of canine IL-12. Cross-reactivity (direct ELISA): ~40 % with recombinant human IL-12 p35/p40 and recombinant feline IL-12 p35/p40, <10 % with recombinant rat IL-12 p35/p40.

Aufreinigung: Affinity purified

Sterilität: 0.2 μm filtered

Immunogen: CHO-derived recombinant canine interleukin 12 p40 (aa23-329, Q28268) and p35 (aa 26-222, Q28267).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Neut, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Neutralization. ELISA (capture): 0.2-0.8 μg/mL. Western Blot: 0.1-0.2 μg/mL. Detection limit for rcaIL-12 is ~50 ng/lane under non-reducing and reducing conditions. Neutralization: The ND 50 was determined to be ~0.1-0.5 μg/mL in the presence of 25 ng/mL of rcaIL-12, using PHA activated human peripheral blood mononuclear cells (PBMCs).

Kommentare:

Target Species of Antibody: Dog

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS, 40-50 % glycerol

Konzentration: Lot specific

Buffer: Lyophilized from 0.2 um filtered solution in PBS, 5 % trehalose

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Details zu IL12

Target: IL12 (Interleukin 12 (IL12))

Andere Bezeichnung: IL12

Hintergrund: Name/Gene ID: IL-12

Synonyms: IL-12

Pathways: JAK-STAT Signalweg, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome