Bcl-2 Antikörper (AA 118-165)

Produktnummer ABIN5693065

Produktdetails anti-Bcl-2 Antikörper

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Bindungsspezifität: AA 118-165

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Bcl-2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Verwendungszweck: Anti-Bcl-2/BCL2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Bcl-2/BCL2 Antibody Picoband® (ABIN5693065). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human Bcl-2 recombinant protein (Position: Q118-E165).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Hockenbery, D., Nunez, G., Milliman, C., Schreiber, R. D., Korsmeyer, S. J. : Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990. 2. Ngan, B.-Y., Chen-Levy, Z., Weiss, L. M., Warnke, R. A., Cleary, M. L. : Expression in non-Hodgkin's lymphoma of the BCL-2 protein associated with the t(14,18) chromosomal translocation. New Eng. J. Med. 318: 1638-1644, 1988.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Bcl-2 Antikörper (ABIN5693065)

Liu, Kuang, Wu, Jin, Sun: "A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo." in: Scientific reports, Vol. 6, pp. 26722, (2018) (PubMed).

Feng, Yan, Zhou, Liang, Liang, Zhao, Dong, Ling: "Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis." in: Oncotarget, Vol. 7, Issue 40, pp. 64575-64588, (2018) (PubMed).

Guo, Lin, Shao, Rong, Zhang: "BMP‑7 suppresses excessive scar formation by activating the BMP‑7/Smad1/5/8 signaling pathway." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1957-1963, (2018) (PubMed).

Chen, Zhu, Yang, Wei, Chen, He, Ji: "Ailanthone induces G2/M cell cycle arrest and apoptosis of SGC‑7901 human gastric cancer cells." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 6821-6827, (2018) (PubMed).

Liang, Zhong, Gong, Wang, Zhu, Liu, Yang et al.: "Fibroblast growth factor 21 protects rat cardiomyocytes from endoplasmic reticulum stress by promoting the fibroblast growth factor receptor 1-extracellular signal‑regulated kinase 1/2 signaling ..." in: International journal of molecular medicine, Vol. 40, Issue 5, pp. 1477-1485, (2018) (PubMed).

An, Liu, She, Wu, Tian, Shi, Hao, Ren, Yang, Lu, Yang, Wu: "Replication of hepatitis E virus in the ovary and promotion of oocyte apoptosis in rabbits infected with HEV-4." in: Oncotarget, Vol. 9, Issue 4, pp. 4475-4484, (2018) (PubMed).

Bai, Yang, Luo: "Effects of 5-hydroxy-4'-nitro-7-propionyloxy-genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells." in: Oncology letters, Vol. 15, Issue 4, pp. 5227-5235, (2018) (PubMed).

Yang, Shi, Soomro, Hu, Du, She: "Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils." in: Frontiers in microbiology, Vol. 9, pp. 460, (2018) (PubMed).

Wang, Li, Yang, Gao, Lin, Wang, Zhou, Hu: "Ginsenoside Rb1 inhibit apoptosis in rat model of Alzheimer's disease induced by Aβ1-40." in: American journal of translational research, Vol. 10, Issue 3, pp. 796-805, (2018) (PubMed).

Li, Wang, Zhou, Shuang, Zhao, Chen: "Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian Granular Cells of Mice." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 2384-2390, (2018) (PubMed).

Yang, Wu, Luo, Zhang: "CCAAT/enhancer binding protein homologous protein knockdown alleviates hypoxia-induced myocardial injury in rat cardiomyocytes exposed to high glucose." in: Experimental and therapeutic medicine, Vol. 15, Issue 5, pp. 4213-4222, (2018) (PubMed).

Qi, Xue, Lv, Sun, Du, Cai, Li, Gu, Wang: "Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochondrial pathway." in: Oncology letters, Vol. 15, Issue 5, pp. 6400-6408, (2018) (PubMed).

Zhou, Wang, Xu, Wan, Qu, Fan, Chen, Sun, Liu: "Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo." in: Oncotarget, Vol. 7, Issue 14, pp. 18620-30, (2017) (PubMed).

Hu, Sun, Zhang, Zhang, Hu: "Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway." in: Bioscience reports, Vol. 36, Issue 3, (2017) (PubMed).

Guo, Wang, Gao, Zhang, Chen, Xu, Hu, Jing, Jing, Li, Wang, Zhu: "Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 22, pp. 3951-3960, (2017) (PubMed).

Hu, Lv, Zhang, Meng: "Comparative Study on the Protective Effects of Salidroside and Hypoxic Preconditioning for Attenuating Anoxia-Induced Apoptosis in Pheochromocytoma (PC12) Cells." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 22, pp. 4082-4091, (2017) (PubMed).

Ustarroz-Cano, Garcia-Pelaez, Cervantes-Yepez, Lopez-Valdez, Fortoul: "Thymic cytoarchitecture changes in mice exposed to vanadium." in: Journal of immunotoxicology, Vol. 14, Issue 1, pp. 9-14, (2017) (PubMed).

Xu, Tan, Jiang, Chen, Lai, Zhang, Liang: "Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure." in: Chinese medicine, Vol. 12, pp. 10, (2017) (PubMed).

Guo, Liu: "Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 2030-2036, (2017) (PubMed).

Shi, Ma, Ren, Liang, Yu, Luo: "Antitumorpharmacological mechanism of the oral liquid of Poriacocos polysaccharide." in: Journal of ethnopharmacology, Vol. 209, pp. 24-31, (2017) (PubMed).

Details zu Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Andere Bezeichnung: BCL2 (BCL2 Produkte)

Hintergrund:

Synonyms: Apoptosis regulator Bcl-2, BCL2

Tissue Specificity: Expressed in a variety of tissues.

Background: Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14,18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Molekulargewicht: 26 kDa

Gen-ID: 596

UniProt: P10415

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling