GBL Antikörper (N-Term)

Produktnummer ABIN5516539

Der Kaninchen Polyklonal Anti-GBL-Antikörper wurde für WB validiert. Er ist geeignet, GBL in Proben von Human zu detektieren.

Kurzübersicht für GBL Antikörper (N-Term) (ABIN5516539)

Target: GBL (G protein beta subunit-like (GBL))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GBL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GBL Antikörper

Bindungsspezifität: N-Term

Sequenz: TRTVQHQDSQ VNALEVTPDR SMIAAAGYQH IRMYDLNSNN PNPIISYDGV

Produktmerkmale: This is a rabbit polyclonal antibody against LST8. It was validated on Western Blot.

Aufreinigung: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human LST8

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu GBL

Target: GBL (G protein beta subunit-like (GBL))

Andere Bezeichnung: LST8

Hintergrund: LST8 is a subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient- poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive.

Alias Symbols: MLST8, GBL, LST8,

Protein Interaction Partner: MTOR, EIF4EBP1, RPTOR, AKT1S1, PRR5L, FKBP8, SUZ12, PRMT1, STAT1, RICTOR, UBC, ATXN1, A2M, DEPTOR, ULK1, RPS6KB1, SQSTM1, Cul4a, Dtl, PRR5, CCT2, KRT85, RHEB,

Protein Size: 326

Gen-ID: 64223

UniProt: Q9BVC4

Pathways: PI3K-Akt Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Autophagie, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effekt