GSK3 beta Antikörper

Produktnummer ABIN4953256

Der Maus-Monoklonal Anti-GSK3 beta-Antikörper wurde für WB, IF, IP und ICC validiert. Er ist geeignet zum Nachweis von GSK3 beta in Proben aus Human, Maus und Ratte. }

Kurzübersicht für GSK3 beta Antikörper (ABIN4953256)

Target: GSK3 beta (GSK3b) (Glycogen Synthase Kinase 3 beta (GSK3b))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GSK3 beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: 2E6-D6-C12

Produktdetails anti-GSK3 beta Antikörper

Aufreinigung: Protein G affinity

Immunogen: A recombinant partial protein was used as the immunogen for this GSK3B antibody.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the GSK3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,ICC/IF: 1:200,Immunoprecipitation: suitable

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Antibody in PBS with 50 % Glycerol and 0.03 % ProClin 300

Lagerung: -20 °C

Informationen zur Lagerung: Store the GSK3B antibody at -20°C.

Details zu GSK3 beta

Target: GSK3 beta (GSK3b) (Glycogen Synthase Kinase 3 beta (GSK3b))

Andere Bezeichnung: GSK3 beta / GSK3B

Hintergrund: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. [UniProt]

UniProt: P49841

Pathways: WNT Signalweg, Hedgehog Signalweg, Fc-epsilon Rezeptor Signalübertragung, Cellular Glucan Metabolic Process, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Autophagie, BCR Signaling, Warburg Effekt