IL-1 beta Antikörper

Produktnummer ABIN476498

Produktdetails anti-IL-1 beta Antikörper

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-1 beta Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Neutralization (Neut)

Aufreinigung: Affinity purified

Immunogen: Human IL1B

Klon: CRM56

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, Neut

Usage: The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1beta (IL-1beta) in combination with the biotin CRM57 (LS-C106945) antibody for detection and recombinant human IL-1beta as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. The Functional Grade Purified CRM56 antibody has been tested for neutralization of IL-1beta bioactivity. The fluorochrome-conjugated CRM56 antibody has been tested by intracellular staining for flow cytometric analysis. The fluorochrome- conjugated CRM56 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining with flow cytometric analysis and can be used at less than or equal to 0.50 μg/million cells/100 μL staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of activated human PBMC and can be used at 20 μL per million cells 100 μL blood.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 150 mM sodium chloride, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freezing and store undiluted.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C, avoid freeze-thaw cycles.

Details zu IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: IL1B (IL1B Produkte)

Hintergrund: Name/Gene ID: IL1B
Family: IL-1

Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta

Gen-ID: 3553

UniProt: P01584

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome