IL12 Antikörper

Produktnummer ABIN465439

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch IL12 in ELISA, WB und Neut. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für IL12 Antikörper (ABIN465439)

Target: IL12 (Interleukin 12 (IL12))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL12 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Neutralization (Neut)

Produktdetails anti-IL12 Antikörper

Spezifität: Human IL-12

Aufreinigung: Immunoaffinity purified

Immunogen: CHO cell derived recombinant Human IL-12.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.01 - 0.02 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-12 (0.3 ng/mL), a concentration of 012-02 μg/mL of this antibody is required. ELISA: To detect hIL-12 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-12 (LS-C104425) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-12. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu IL12

Target: IL12 (Interleukin 12 (IL12))

Andere Bezeichnung: IL12 / IL-12

Hintergrund: Name/Gene ID: IL-12

Synonyms: IL-12

Pathways: JAK-STAT Signalweg, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome