PDK2 Antikörper (C-Term)

Produktnummer ABIN391037

Der Kaninchen Polyklonal Anti-PDK2-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, PDK2 in Proben von Human zu detektieren. Es sind 10+ Publikationen verfügbar.

Kurzübersicht für PDK2 Antikörper (C-Term) (ABIN391037)

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB03037

Produktdetails anti-PDK2 Antikörper

Bindungsspezifität: AA 378-407, C-Term

Homologie: M, Rat

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-407 amino acids from the C-terminal region of human PDK2.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-PDK2 Antikörper (ABIN391037)

Hurd, Collins, Abakumova, Chouchani, Baranowski, Fearnley, Prime, Murphy, James: "Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species." in: The Journal of biological chemistry, Vol. 287, Issue 42, pp. 35153-60, (2012) (PubMed).

Love, LeBlanc, Inglis, Bradley, Choptiany, Heigenhauser, Peters: "The relationship between human skeletal muscle pyruvate dehydrogenase phosphatase activity and muscle aerobic capacity." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 111, Issue 2, pp. 427-34, (2011) (PubMed).

Dunford, Herbst, Jeoung, Gittings, Inglis, Vandenboom, LeBlanc, Harris, Peters: "PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 300, Issue 6, pp. R1487-93, (2011) (PubMed).

Nicholls, Chu, Abbruzzese, Tremblay, Hardy: "Mechanism of a genetically encoded dark-to-bright reporter for caspase activity." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24977-86, (2011) (PubMed).

McClenaghan, Scullion, Mion, Hewage, Malthouse, Flatt, Newsholme, Brennan: "Prolonged L-alanine exposure induces changes in metabolism, Ca(2+) handling and desensitization of insulin secretion in clonal pancreatic beta-cells." in: Clinical science (London, England : 1979), Vol. 116, Issue 4, pp. 341-51, (2009) (PubMed).

Sun, Zhou, Chang, McFate, Verma, Califano: "Mitochondrial mutations contribute to HIF1alpha accumulation via increased reactive oxygen species and up-regulated pyruvate dehydrogenease kinase 2 in head and neck squamous cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 2, pp. 476-84, (2009) (PubMed).

Richards, Sardella, Schulte: "Regulation of pyruvate dehydrogenase in the common killifish, Fundulus heteroclitus, during hypoxia exposure." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 295, Issue 3, pp. R979-90, (2008) (PubMed).

Zhang, Ma, Sadana, Chowdhury, Gaillard, Wang, McDonnell, Unterman, Elam, Park: "Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression." in: The Journal of biological chemistry, Vol. 281, Issue 52, pp. 39897-906, (2006) (PubMed).

Xu, Han, Epstein, Liu: "Regulation of PDK mRNA by high fatty acid and glucose in pancreatic islets." in: Biochemical and biophysical research communications, Vol. 344, Issue 3, pp. 827-33, (2006) (PubMed).

Churchill, Murriel, Chen, Mochly-Rosen, Szweda: "Reperfusion-induced translocation of deltaPKC to cardiac mitochondria prevents pyruvate dehydrogenase reactivation." in: Circulation research, Vol. 97, Issue 1, pp. 78-85, (2005) (PubMed).

Details zu PDK2

Target: PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2))

Andere Bezeichnung: PDK2

Hintergrund: PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Molekulargewicht: 46154

Gen-ID: 5164

NCBI Accession: NP_001186827, NP_001186828, NP_002602

UniProt: Q15119

Pathways: PI3K-Akt Signalweg, RTK Signalweg, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt