AKT1 Antikörper (pSer473)

Produktnummer ABIN389819

Produktdetails anti-AKT1 Antikörper

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Bindungsspezifität: pSer473

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Dot Blot (DB)

Homologie: M

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Phospho-AKT1-pS473 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S473 of human AKT1.

Klon: RB13096

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. DB: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-AKT1 Antikörper (ABIN389819)

Hu, Zhou, Zhao, Wu: "Integrin α6/Akt/Erk signaling is essential for human breast cancer resistance to radiotherapy." in: Scientific reports, Vol. 6, pp. 33376, (2018) (PubMed).

Li, Miao, Shan, Liu, Li, Zhao, Jia: "MiR-106b and miR-93 regulate cell progression by suppression of PTEN via PI3K/Akt pathway in breast cancer." in: Cell death & disease, Vol. 8, Issue 5, pp. e2796, (2018) (PubMed).

Zhao, Shan, Liu, Li, Jia: "Functional screen analysis reveals miR-3142 as central regulator in chemoresistance and proliferation through activation of the PTEN-AKT pathway in CML." in: Cell death & disease, Vol. 8, Issue 5, pp. e2830, (2018) (PubMed).

Miao, Zheng, Li, Su, Zhao, Zhou, Jia: "MicroRNA-130b targets PTEN to mediate drug resistance and proliferation of breast cancer cells via the PI3K/Akt signaling pathway." in: Scientific reports, Vol. 7, pp. 41942, (2017) (PubMed).

Li, Lu, Yu, Han, Wang, Mao, Shen, Wang, Tang, Li, Song: "miR-221/222 promote cancer stem-like cell properties and tumor growth of breast cancer via targeting PTEN and sustained Akt/NF-κB/COX-2 activation." in: Chemico-biological interactions, Vol. 277, pp. 33-42, (2017) (PubMed).

Ren, Jia, Li, Gong, Liu, Zhang, Wang, Zhao: "ST6GalNAcII mediates the invasive properties of breast carcinoma through PI3K/Akt/NF-?B signaling pathway." in: IUBMB life, Vol. 66, Issue 4, pp. 300-8, (2014) (PubMed).

Li, Jia, Ren, Liu, Gong, Wang, Zhang, Zhao: "Axl mediates tumor invasion and chemosensitivity through PI3K/Akt signaling pathway and is transcriptionally regulated by slug in breast carcinoma." in: IUBMB life, Vol. 66, Issue 7, pp. 507-18, (2014) (PubMed).

Details zu AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Andere Bezeichnung: AKT1 (AKT1 Produkte)

Hintergrund: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Molekulargewicht: 55686

Gen-ID: 207

NCBI Accession: NP_001014431, NP_001014432, NP_005154

UniProt: P31749

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt