GTPase NRas Antikörper (Internal Region)

Produktnummer ABIN375000

Dieses Anti-GTPase NRas-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von GTPase NRas in WB und IHC (p). Geeignet für Human, Ratte, Maus und Hund.

Kurzübersicht für GTPase NRas Antikörper (Internal Region) (ABIN375000)

Target: GTPase NRas (NRAS)

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GTPase NRas Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GTPase NRas Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-RMKKLNSSDD GTQ

Spezifität: This antibody detects NRAS.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Synthetic peptide corresponding to internal sequence of Human NRAS (NP_002515.1). Genename: NRAS

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.01-0.03 μg/mL. Approx.21 kDa band observed in Human lysates of cell lineA431. In transfected HEK293 transiently expressing NRAS bands of approx.21 kDa and21 kDa are observed. These bands are not observed in the non-transfected HEK293. Immunohistochemistry: 2-3 μg/mL. In paraffin embedded Human Small Intestine shows stainingof cytoplasm and membrane of enterocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GTPase NRas

Target: GTPase NRas (NRAS)

Andere Bezeichnung: NRAS

Hintergrund: NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.Synonyms: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog

Molekulargewicht: 21 kDa (NP_002515.1).

Gen-ID: 4893, 9606

UniProt: P01111

Pathways: p53 Signalweg, MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling