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GTPase NRas Antikörper (Internal Region)

Verified NRAS Reaktivität: Human WB, ELISA Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN302138
  • Target Alle GTPase NRas (NRAS) Antikörper anzeigen
    GTPase NRas (NRAS)
    Bindungsspezifität
    • 11
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 44
    • 20
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 43
    • 12
    Ziege
    Klonalität
    • 41
    • 14
    Polyklonal
    Konjugat
    • 35
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GTPase NRas Antikörper ist unkonjugiert
    Applikation
    • 44
    • 17
    • 8
    • 7
    • 7
    • 7
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Verwendungszweck
    NRAS
    Sequenz
    RMKKLNSSDD GTQ
    Kreuzreaktivität
    Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.
    Isotyp
    IgG
  • Applikationshinweise

    Western Blot: Approx. 21-22 kDa band observed in lysates of cell line MCF7 (calculated MW of 21.2 kDa according to NP_002515.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Kommentare

    Immunofluorescence: A customer obtained weak cytoplasmic staining in MCF7 cells at 10μg/ml.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    GTPase NRas (NRAS)
    Andere Bezeichnung
    NRAS (NRAS Produkte)
    Hintergrund
    NRAS , neuroblastoma RAS viral (v-ras) oncogene homolog , N-ras , NRAS1, N-ras protein part 4 , v-ras neuroblastoma RAS viral oncogene homolog
    Gen-ID
    4893, 18176, 24605
    NCBI Accession
    NP_002515
    Pathways
    p53 Signalweg, MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
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