Parkin Antikörper (pSer101)

Produktnummer ABIN372697

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Parkin in WB. Er zeigt eine Reaktivität gegenüber Human, Rind (Kuh) und Non-Human Primate.

Kurzübersicht für Parkin Antikörper (pSer101) (ABIN372697)

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reaktivität: Human, Rind (Kuh), Non-Human Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Parkin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Parkin Antikörper

Bindungsspezifität: pSer101

Spezifität: This antibody recognizes the ~52k Parkin protein phosphorylated at Ser101. Immunolabeling of the parkin band is absent in parkin S101 mutants.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine and non-Human Primates.
Species reactivity (tested):Human.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser101 of human Parkin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu Parkin

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Andere Bezeichnung: PARK2 / Parkin

Hintergrund: Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson's disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner et al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser101 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).Synonyms: AR-JP, E3 ubiquitin-protein ligase parkin, LPRS2, PDJ, PRKN, Parkinson disease 2, Parkinson juvenile disease protein 2

Gen-ID: 5071

NCBI Accession: NP_004553

UniProt: O60260

Pathways: Autophagie, Ubiquitin Proteasome Pathway