Parkin Antikörper (AA 23-416)
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- Target Alle Parkin (PARK2) Antikörper anzeigen
- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
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Bindungsspezifität
- AA 23-416
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Parkin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Verwendungszweck
- Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase parkin(PARK2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
- Kreuzreaktivität (Details)
- No cross reactivity with other proteins.
- Produktmerkmale
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Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase parkin(PARK2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: parkin RBR E3 ubiquitin protein ligase
Protein Name: E3 ubiquitin-protein ligase parkin - Aufreinigung
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.
- Isotyp
- IgG
- Top Product
- Discover our top product PARK2 Primärantikörper
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- Applikationshinweise
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Parkin is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
- Andere Bezeichnung
- PARK2 (PARK2 Produkte)
- Synonyme
- CG10523 antikoerper, Dmel\\CG10523 antikoerper, Dpark antikoerper, SD01679 antikoerper, dpk antikoerper, AR-JP antikoerper, LPRS2 antikoerper, PDJ antikoerper, PRKN antikoerper, Park antikoerper, Prkn antikoerper, si:ch211-123f21.1 antikoerper, zgc:112390 antikoerper, pdr-1 antikoerper, PARK2 antikoerper, parkin antikoerper, parkin RBR E3 ubiquitin protein ligase antikoerper, Parkinson disease (autosomal recessive, juvenile) 2, parkin antikoerper, park antikoerper, PRKN antikoerper, Prkn antikoerper, prkn antikoerper, CpipJ_CPIJ014867 antikoerper, Park2 antikoerper
- Hintergrund
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Parkin is a RING domain-containing E3 ubiquitin ligase involved in proteasome-dependent degradation of proteins. It is mapped to 6q26. This gene is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy. Parkin is expressed in neuronal processes and cell bodies of neurons, but not glial cells, in the midbrain, basal ganglia, cerebral cortex, and cerebellum. Parkin assimilated with actin filaments, suggesting that it is a cytoskeletal-associated protein. Parkin is identified as a transcriptional repressor of p53 independent of its ubiquitin ligase function. It also has been found that parkin was associated physically with mitochondrial DNA (mtDNA) in proliferating as well as in differentiated SH-SY5Y neuroblastoma cells.
Synonyms: AR JP antibody|E3 ubiquitin ligase antibody|E3 ubiquitin protein ligase parkin antibody|E3 ubiquitin-protein ligase parkin antibody|FRA6E antibody|LPRS 2 antibody|LPRS2 antibody|PARK 2 antibody|PARK2 antibody|Parkin 2 antibody|Parkinson disease (autosomal recessive juvenile) 2 antibody|Parkinson disease (autosomal recessive, juvenile) 2, parkin antibody|Parkinson disease protein 2 antibody|Parkinson juvenile disease protein 2 antibody|Parkinson protein 2 E3 ubiquitin protein ligase antibody|Parkinson protein 2, E3 ubiquitin protein ligase (parkin) antibody|PDJ antibody|PRKN 2 antibody|PRKN antibody|PRKN2 antibody|PRKN2_HUMAN antibody|Ubiquitin E3 ligase PRKN antibody - Gen-ID
- 5071
- UniProt
- O60260
- Pathways
- Autophagie, Ubiquitin Proteasome Pathway
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