p53 Antikörper (pSer392)

Produktnummer ABIN372695

Der Kaninchen Polyklonal Anti-p53-Antikörper wurde für WB validiert. Er ist geeignet, p53 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für p53 Antikörper (pSer392) (ABIN372695)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-p53 Antikörper

Bindungsspezifität: pSer392

Spezifität: This antibody recognizes the ~53k p53 protein phosphorylated at Ser392. The immunolabeling is completely eliminated by lambda-phosphatase treatment.

Kreuzreaktivität (Details): Species reactivity (expected):Human and non-Human Primates.
Species reactivity (tested):Rat.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser392 of human p53.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53 (TP53)

Hintergrund: P53 has a well established role in blocking the proliferative action of damaged cells and acting in essence as an anticancer agent (Sharpless and DePinho, 2002, Yin et al., 1992). It has been called the guardian of the genome (Lane, 1992). Phosphorylation of Ser392 in p53 is associated with formation of human tumors (Saito et al., 2003, Pise-Masison et al., 1998, Kim et al., 2004). In addition, p53 has been linked to effects of aging and oxidative stress (Sharpless and DePinho, 2002). An increase in p53 has also been linked to deficits in LTP and learning and memory (Jiang et al., 1998).Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Gen-ID: 24842

NCBI Accession: NP_112251

UniProt: P10361

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt