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p53 Antikörper (AA 4-8)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch p53 in WB. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 2+ Publikationen erwähnt.
Produktnummer ABIN362642

Kurzübersicht für p53 Antikörper (AA 4-8) (ABIN362642)

Target

Alle p53 (TP53) Antikörper anzeigen
p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität

  • 968
  • 279
  • 232
  • 143
  • 75
  • 74
  • 60
  • 40
  • 10
  • 9
  • 9
  • 6
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Maus

Wirt

  • 670
  • 324
  • 5
  • 2
  • 2
Kaninchen

Klonalität

  • 575
  • 427
  • 1
Polyklonal

Konjugat

  • 527
  • 51
  • 32
  • 20
  • 19
  • 18
  • 17
  • 17
  • 17
  • 17
  • 17
  • 17
  • 14
  • 14
  • 14
  • 14
  • 14
  • 14
  • 14
  • 14
  • 14
  • 12
  • 12
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 10
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Dieser p53 Antikörper ist unkonjugiert

Applikation

  • 732
  • 323
  • 287
  • 241
  • 212
  • 142
  • 133
  • 129
  • 122
  • 119
  • 80
  • 64
  • 35
  • 20
  • 17
  • 12
  • 10
  • 9
  • 8
  • 6
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Bindungsspezifität

    • 39
    • 38
    • 38
    • 31
    • 31
    • 30
    • 26
    • 26
    • 24
    • 22
    • 21
    • 20
    • 19
    • 19
    • 19
    • 18
    • 18
    • 16
    • 14
    • 12
    • 9
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    AA 4-8

    Spezifität

    The antibody detects endogenous level of total p53 protein.

    Aufreinigung

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    Immunogen

    Peptide sequence around AA 4-8 (P-Q-S-D-P) derived from Human p53. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

    Isotyp

    IgG
  • Applikationshinweise

    Western blotting: 1:500-1:1000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Ji, Ding, Hawke, Xing, Jiang, Mills, Lu: "AKT-dependent phosphorylation of Niban regulates nucleophosmin- and MDM2-mediated p53 stability and cell apoptosis." in: EMBO reports, Vol. 13, Issue 6, pp. 554-60, (2012) (PubMed).

    Gao, Zhai, Guan, Zheng: "Nordihydroguaiaretic acid inhibits growth of cervical cancer SiHa cells by up-regulating p21." in: Oncology letters, Vol. 2, Issue 1, pp. 123-128, (2012) (PubMed).

  • Target

    p53 (TP53) (Tumor Protein P53 (TP53))

    Andere Bezeichnung

    p53

    Hintergrund

    P53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes

    Molekulargewicht

    53 kDa

    NCBI Accession

    NP_000537

    UniProt

    P04637

    Pathways

    p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt
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