AKT1 Antikörper (pSer473)

Produktnummer ABIN361978

Produktdetails anti-AKT1 Antikörper

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Bindungsspezifität: pSer473

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: The antibody detects endogenous level of Akt only when phosphorylated at serine 473.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer473 (Q-F-S (p) -Y-S) derived from Human Akt. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-AKT1 Antikörper (ABIN361978)

Wang, Li, Zhang, Li, Wu, Yang, Nie, Wu, Shi, Fan: "MicroRNA-19a/b regulates multidrug resistance in human gastric cancer cells by targeting PTEN." in: Biochemical and biophysical research communications, Vol. 434, Issue 3, pp. 688-94, (2013) (PubMed).

Jia, Xu, Liu, Guo, Zhang, Lu, Xu, Wei, Zhu, Zhang, Zhang, Sun, You: "Identification, design and bio-evaluation of novel Hsp90 inhibitors by ligand-based virtual screening." in: PLoS ONE, Vol. 8, Issue 4, pp. e59315, (2013) (PubMed).

Ma, Li, Wang, Gong, Zhang, Li, Ji, Liu, Chen, Xiang: "Atrial natriuretic peptide suppresses Th17 development through regulation of cGMP-dependent protein kinase and PI3K-Akt signaling pathways." in: Regulatory peptides, Vol. 181, pp. 9-16, (2013) (PubMed).

Schubert, Coupland, Culibrk, Goodrich, Devine: "Riboflavin and ultraviolet light treatment of platelets triggers p38MAPK signaling: inhibition significantly improves in vitro platelet quality after pathogen reduction treatment." in: Transfusion, Vol. 53, Issue 12, pp. 3164-73, (2013) (PubMed).

Ning, Huang, Dong, Sun, Zhang, Xu, Li: "5-Aza-2'-deoxycytidine inhibited PDGF-induced rat airway smooth muscle cell phenotypic switching." in: Archives of toxicology, Vol. 87, Issue 5, pp. 871-81, (2013) (PubMed).

Shen, Song, An, Li, Wu, Ruan, Hu, Hu: "The use of hollow mesoporous silica nanospheres to encapsulate bortezomib and improve efficacy for non-small cell lung cancer therapy." in: Biomaterials, Vol. 35, Issue 1, pp. 316-26, (2013) (PubMed).

Zhang, Hou, Cui, Guo, Shi, Yang, Dai: "Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity." in: Toxicology letters, Vol. 218, Issue 1, pp. 61-9, (2013) (PubMed).

Callén, Moreno, Barroso-Chinea, Moreno-Delgado, Cortés, Mallol, Casadó, Lanciego, Franco, Lluis, Canela, McCormick: "Cannabinoid receptors CB1 and CB2 form functional heteromers in brain." in: The Journal of biological chemistry, Vol. 287, Issue 25, pp. 20851-65, (2012) (PubMed).

Luan, Zhao, Zhao, Zhu, Xiu, Ji: "Hydrogen sulfide postconditioning protects isolated rat hearts against ischemia and reperfusion injury mediated by the JAK2/STAT3 survival pathway." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], Vol. 45, Issue 10, pp. 898-905, (2012) (PubMed).

Han, Quirion, Zheng: "WITHDRAWN: Glutamate Attenuates IGF-1 Receptor Signaling via NR2B containing NMDA Receptors 3 and neuronal Nitric Oxide Synthase." in: Biochemical and biophysical research communications, (2011) (PubMed).

Yu, Qin, He, Wang, Fu, Liu, Guo, Liang, Dou, Han: "Canonical notch pathway protects hepatocytes from ischemia/reperfusion injury in mice by repressing reactive oxygen species production through JAK2/STAT3 signaling." in: Hepatology (Baltimore, Md.), Vol. 54, Issue 3, pp. 979-88, (2011) (PubMed).

Ma, Yao, Zhang, Lin, Wang, Wu, Sun, Chen: "The effects of artesunate on the expression of EGFR and ABCG2 in A549 human lung cancer cells and a xenograft model." in: Molecules (Basel, Switzerland), Vol. 16, Issue 12, pp. 10556-69, (2011) (PubMed).

Vendrell, Molero, González, Pérez-Capote, Lluis, McCormick, Franco, Cortés, Casadó, Albericio, Royo: "Biotin ergopeptide probes for dopamine receptors." in: Journal of medicinal chemistry, Vol. 54, Issue 4, pp. 1080-90, (2011) (PubMed).

Guo, Yang, Lu, Li, Wan, Long, Li, Feng, Li: "Rb1 postconditioning attenuates liver warm ischemia-reperfusion injury through ROS-NO-HIF pathway." in: Life sciences, Vol. 88, Issue 13-14, pp. 598-605, (2011) (PubMed).

Li, Liu: "The novel squamosamide derivative FLZ enhances BDNF/TrkB/CREB signaling and inhibits neuronal apoptosis in APP/PS1 mice." in: Acta pharmacologica Sinica, Vol. 31, Issue 3, pp. 265-72, (2010) (PubMed).

Lin, Yan, Zhang, Yuan, Li, Qiao, Wang, Sun: "ER-alpha36, a variant of ER-alpha, promotes tamoxifen agonist action in endometrial cancer cells via the MAPK/ERK and PI3K/Akt pathways." in: PLoS ONE, Vol. 5, Issue 2, pp. e9013, (2010) (PubMed).

Wang, Xu, Wu, Guan, Cui, Lei, Zhang, Mou, Sun, Dai: "Neuregulin-1 enhances differentiation of cardiomyocytes from embryonic stem cells." in: Medical & biological engineering & computing, Vol. 47, Issue 1, pp. 41-8, (2009) (PubMed).

Zhou, Zhao, Zhang: "Inhibition of uncoupling protein 2 by genipin reduces insulin-stimulated glucose uptake in 3T3-L1 adipocytes." in: Archives of biochemistry and biophysics, Vol. 486, Issue 1, pp. 88-93, (2009) (PubMed).

Ren, Li, Xiao, Yan, Yang, Wang, Guo, Wang: "Endocrine glands-derived vascular endothelial growth factor protects pancreatic cancer cells from apoptosis via upregulation of the myeloid cell leukemia-1 protein." in: Biochemical and biophysical research communications, Vol. 386, Issue 1, pp. 35-9, (2009) (PubMed).

Kim, Lee, Kim, Bahk: "A Proteomic approach for protein-profiling the oncogenic ras induced transformation (H-, K-, and N-Ras) in NIH/3T3 mouse embryonic fibroblasts." in: Proteomics, Vol. 8, Issue 15, pp. 3082-93, (2008) (PubMed).

Details zu AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Andere Bezeichnung: Akt (AKT1 Produkte)

Hintergrund: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

Molekulargewicht: 60 kDa

NCBI Accession: NP_001014

UniProt: P31749

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt